prenatal sex determination using amplification of y-specific sequences in maternal plasma
نویسندگان
چکیده
background: fetal dna has been found in maternal plasma. diagnosis of fetal gender using maternal plasma has been attempted in an effort to develop a new noninvasive method of prenatal diagnosis. methods: we extracted dna from plasma of 30 pregnant woman, three men, three non-pregnant women, and performed a sensitive y-pcr assay to detect circulating male fetal dna from women bearing male fetuses. fetus-derived y sequences were detected using 40 cycles of pcr carried out for each dna extract. the pcr products were analyzed by 8% acrylamide gel electrophoresis and ethidium bromide staining, and the results were compared after delivery. findings:y sequences were detected in three control men and 14 pregnant woman who had a male fetus. two samples from an eleven weeks and 19 weeks women bearing male fetuses gave negative result. none of 8 woman bearing female fetuses and none of the three non-pregnant control women gave a positive y signal. in one sample from a 12 weeks woman bearing female fetus y sequences was detected. interpretation: conventional pcr analysis of maternal plasma can be used to diagnose fetal gender after 19 weeks. prenatal sex determination befor 15 weeks needs more sensitive methods such as quantitative pcr.
منابع مشابه
An Effective Method for Detecting Y-chromosome Specific Sequences of Circulating Fetal DNA in Maternal Plasma During the First-trimester
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متن کامل
comparative in silico study of sex-determining region y (sry) protein sequences involved in sex-determining
background: the sry gene (sry) provides instructions for making a transcription factor called the sex-determining region y protein. the sex-determining region y protein causes a fetus to develop as a male. in this study, sry of 15 spices included of human, chimpanzee, dog, pig, rat, cattle, buffalo, goat, sheep, horse, zebra, frog, urial, dolphin and killer whale were used for determine of bioi...
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عنوان ژورنال:
علومجلد ۱۸، شماره ۴۹، صفحات ۴۰۱-۴۰۹
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